Journal: Journal of Neuroscience

Article Title: Vascular Remodeling versus Amyloid -Induced Oxidative Stress in the Cerebrovascular Dysfunctions Associated with Alzheimer's Disease

doi: 10.1523/jneurosci.4031-05.2005

Figure Lengend Snippet: Figure6. NitrosativestressinthecerebralcortexofAPP andTGF mice.Nitrotyrosineimmunoreactivityisupregulatedin the vessel walls of young APP mice, whereas it is distributed in the neuropil and around intraparenchymal blood vessels in 12-month-old APP mice. As shown in the adjacent panels, nitrotyrosine immunoreactivity (red) is mainly located in CD11b- immunopositive microglial processes (green; the merge with nitrotyrosine is yellow) and cells (open arrows), but some neurons are also nitrotyrosine immunopositive (small arrows). In TGF mice, nitrotyrosine immunoreactivity is primarily distributed to glial elements in both wild-type (WT) and transgenic mice. Scale bar, 75 m. mo, Month.

Article Snippet: Changes in pial and/or intracortical microvessels in transgenic mice (perfusion-fixed brains) and AD patients (50- m-thick sections) were assessed by immunostaining for the following markers: SOD2 (rabbit anti-MnSOD; 1:400; Stressgen), nitrosative stress (mouse anti-nitrotyrosine; 1:1000; Upstate Biotechnology, Lake Placid, NY), basement membrane proteins collagen-I (goat anti- collagen type I; 1:400; Southern Biotechnology, Birmingham, AL) and collagen-IV (goat anti-collagen type IV; 1: 400; Chemicon), and astroglial (GFAP, rabbit anti-GFAP; 1:300; Dako, Glostrup, Denmark) or microglial (rat anti-CD11b; 1:100; Serotec, Raleigh, NC) activation.

Techniques: Transgenic Assay

Journal: Molecular Metabolism

Article Title: Loss of Tbk1 kinase activity protects mice from diet-induced metabolic dysfunction

doi: 10.1016/j.molmet.2018.06.007

Figure Lengend Snippet: Tbk1 Δ/ Δ mice have less inflammation relative to Tbk1 +/ + mice on HFD . (A) Representative 20x images and quantification of cd11b/c stained subcutaneous white adipose tissue from Tbk1 Δ/Δ and Tbk1 +/+ mice after 10 weeks on ND or HFD (n = 4–8 mice/group). Scale bar indicates 200 μM and ‘CLS’ refers to crown like structures. (B) mRNA expression of genes encoding CD11c, F4/80, TNFα, IL-6 and IL-12 in subcutaneous WAT of Tbk1 Δ/Δ and Tbk1 +/+ mice fed with HFD as indicated (n = 4–6 mice/group). (C) Liver tissue lysates from 14-week-old HFD-fed Tbk1 Δ/Δ and Tbk1 +/+ mice were immunoblotted with antibodies against IL-6 and IL-1β. β-actin and GAPDH were used as internal loading controls. Indicated cytokines from WAT (D) and liver tissue (E) lysates of HFD-fed Tbk1 +/+ and Tbk1 Δ/Δ mice were measured by Bio-Rad multiplex array (n = 4–8 mice/group for WAT, n = 8–12 mice/group for liver). All mice are from 129S5 background. Results are representative of mean +/− SEM. Statistical analysis by Student's t -test. * p < 0.05, ** p < 0.01.

Article Snippet: Sections for immunohistochemical analysis were blocked with 20% aquablock and incubated with rabbit anti-cd11b/c (Novus Biologicals, NB110-40766) in blocking solution (5% BSA in TBS with 0.05% tween) at 4 °C overnight.

Techniques: Staining, Expressing, Multiplex Assay

Journal: Restorative neurology and neuroscience

Article Title: Progesterone treatment shows greater protection in brain vs. retina in a rat model of middle cerebral artery occlusion: Progesterone receptor levels may play an important role

doi: 10.3233/RNN-160672

Figure Lengend Snippet: Detailed information on antibodies used in this study

Article Snippet: rat anti-CD11b , 128 kDa , 1 : 1000 , Serotec , MCA 275.

Techniques: Molecular Weight

Journal: Frontiers in Oncology

Article Title: Prognostic stratification based on the levels of tumor-infiltrating myeloid-derived suppressor cells and PD-1/PD-L1 axis in locally advanced rectal cancer

doi: 10.3389/fonc.2022.1018700

Figure Lengend Snippet: Representative immunofluorescence images of two cases with (A−D) high (upper row) and (E−H) low (lower row) number of tumor-infiltrating MDSCs. Based on the expression of (A, E) CD33, (B, F) CD11b, and (C, G) DAPI, (D, H) the merged triple immunofluorescence results were assessed. Cells with yellow color were merged from red and green. yellow arrows: CD11b + CD33 - cells; white arrows: CD11b + CD33 + cells; Scale bar: 20 μm. MDSC, myeloid-derived suppressor cell; DAPI, 4,6-diamidino-2-phenylindole.

Article Snippet: Subsequently, nonspecific antigens were blocked using 0.2% bovine serum albumin in PBS for 1 h. For the double-staining of CD11b and CD33, a cocktail of primary antibodies including anti-CD11b (#NB110-89474, Novus Biologics; 1:800) and anti-CD33 (#MAB11371-100, R&D systems; 1:500), was incubated in blocking solution overnight at 4°C.

Techniques: Immunofluorescence, Expressing, Derivative Assay